INSTRUCTIONS :
1. Check the expiry date on the bottom of the container.
2. Use fresh urine not older than four hours. Mix well before testing, do not centrifuge.
3. Remove a test strip from the container and REPLACE THE CAP IMMEDIATELY
4. Briefly (no longer than one second) dip the entire test strip into the urine and remove immediately.
5. Blot one edge of the test strip on the absorbent tissue to remove excess urine.
6. Reading time for specific parameters are very much important for best results. Read Glucose & Bilirubin at 30 seconds after dipping; Ketone at 40 seconds. The pH and protein may be read immediately or at anytime up to 1 minute after dipping.
PROTEIN (ALBUMIN) :
The test incorporates a pH indicator which changes colour in the presence of protein. Any green colour that develops is due to the presence of protein in the urine. The test is highly sensitive for albumin, and less sensitive to hemoglobin, globulin, and mucoprotein. Normal urine of high specific gravity may cause higher than normal readings, as well highly buffered urine, alkaline urine (pH>9), and urine containing residues of chlorhexidine or quartenary ammonium compounds.
• Sensitivity : 10 mg/dl albumin
• Active reagent : Tetrabromophenol blue (1% w/w)
ACETOACETIC ACID (KETONES) :
When acetoacetic acid reacts with nitroprusside, a colour change develops covering the range from beige (negative) to marron (positive). The test does not react with acetone or β- hydroxy butyrate. Urine samples of low pH and high specifice gravity, and other substances containing sulfhydryl groups occasionally give reactions up to and including trace (+).
1. Sensitivity : 0.5 mmol/l acetoacetic acid
2. Active reagent : Nitropruside ( 7.5% w/w)
GLUCOSE :
A double sequential enzymatic reaction, involving glucose oxidase and peroxidase in the presence of an indicator is responsible for the colour change. Ketones or other metabolic substances should not produce false positive, as the test is highly specific for glucose. Sensitivity may decrease with urine of high specific gravity (>1.030) and with ascorbic acid concentrations of > 1.4 mml/l.
• Sensitivity : 2.0 mmol/l glucose
• Active reagents : Glucose oxidase (100 U/ml), peroxidase (100 U/ml)
IMPORTANT NOTES :
1. As with similar urine tests, this product is adversely affected by light and humidity. Exposure to these factors will decrease both shelf life and accuracy. Therefore the container must at all time be promptly and tightly closed with the desiccant filled cap to prevent premature reaction and damage to the test strips.
2. After dipping the test strip into a urine sample, blot the one side of the test strip on absorbent paper or tissue. This prevents running of reagents and dyes between test patches and ensures optimal performance.
3. In general, therapy should not be initiated based on the findings of a single result but rather in conjunction with a proper clinical assessment.
The product is intended for in vitro diagnostic use, and should not be used to analyse body fluids other than urine. Keep out of reach of children.
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